Document Type

Article

Publication Title

Limnology and Oceanography: Methods

Rights and Access Note

This Item is protected by copyright and/or related rights. You are free to use this item in any way that is permitted by copyright and related rights legislation that applies to your use. Rights assessment remains the responsibility of the researcher. In addition, no permission is required from the rights-holder(s) for non-commercial uses.

Publication Date

6-1-2017

First Page

572

Last Page

585

Issue Number

6

Volume Number

15

Abstract/ Summary

Chlorophyll fluorometers provide the largest in situ global data set for estimating phytoplankton biomass because of their ease of use, size, power consumption, and relatively low price. While in situ chlorophyll a (Chl) fluorescence is proxy for Chl a concentration, and hence phytoplankton biomass, there exist large natural variations in the relationship between in situ fluorescence and extracted Chl a concentration. Despite this large natural variability, we present here a global validation data set for the WET Labs Environmental Characterization Optics (ECO) series chlorophyll fluorometers that suggests a factor of 2 overestimation in the factory calibrated Chl a estimates for this specific manufacturer and series of sensors. We base these results on paired High Pressure Liquid Chromatography (HPLC) and in situ fluorescence match ups for which non-photochemically quenched fluorescence observations were removed. Additionally, we examined matchups between the factory-calibrated in situ fluorescence and estimates of chlorophyll concentration determined from in situ radiometry, absorption line height, NASA's standard ocean color algorithm as well as laboratory calibrations with phytoplankton monocultures spanning diverse species that support the factor of 2 bias. We therefore recommend the factor of 2 global bias correction be applied for the WET Labs ECO sensors, at the user level, to improve the global accuracy of chlorophyll concentration estimates and products derived from them. We recommend that other fluorometer makes and models should likewise undergo global analyses to identify potential bias in factory calibration.

Citation/Publisher Attribution

Xing X., H. Claustre, E. Boss, C. Roesler, E. Organelli, A. Pauteau, M. Barbieux, and F. D'Ortenzio. 2016. Correction of profiles of in-situ chlorophyll fluorometry for the contribution of fluorescence originating from non-algal matter. Limnology and Oceanography: Methods, 3, doi: 10.1002/lom3.10144

Publisher Statement

© 2017 The Authors Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography

DOI

10.1002/lom3.10185

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publisher's version of the published document

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In Copyright - Educational Use Permitted.