Date of Award
Level of Access Assigned by Author
Master of Science (MS)
Second Committee Member
Third Committee Member
Additional Committee Members
Two-photon microscopy (TPM) is a powerful, versatile imaging modality for the study of biological systems. This thesis overviews the relevant physics involved in TPM, design considerations and process of constructing a home-built, two-photon microscope, and provides a set of procedures to operate the system. Furthermore, this work explores several applications of TPM through the study of single-cell metabolism and imaging the cellular-material interface. Explored in particular depth was the imaging of cellulose nanofiber (CNF) materials, with the goal of understanding the three-dimensional nature of fibroblast cell growth when embedded within the materials. This work uncovered several optical properties of CNF, and resulted in the creation of a novel method for imaging three-dimensional cellular growth into CNF materials; resolving fine material and biological structures without perturbing the native environment.
Breeding, William P., "Design, Construction and Application of a Home-Built, Two-Photon Microscope" (2019). Electronic Theses and Dissertations. 3077.