Advancing Development of Porphyra umbilicalis as a Red Algal Model System and Aquaculture Crop
Date of Award
Level of Access Assigned by Author
Masters of Science in Bioscience (MSB)
Susan H. Brawley
Second Committee Member
Third Committee Member
Additional Committee Members
The marine red alga Porphyra umbilicalis (Rhodophyta, Bangiaceae) has ideal traits to allow it to become a model organism, including its economic value, reproduction in the northwestern Atlantic through asexual neutral spores (NS), and availability of fully-sequenced nuclear and organelle genomes. Research on the bacterial component of the Porphyra microbiome is ongoing. To advance model organism development and support microbial studies, data on natural reproductive trends and early embryonic development are needed, along with a system for genetic transformation, and ways of visualizing the attached microbial community. To meet these needs, two years of phenological data were analyzed, revealing seasonal reproductive trends and some location-based effects. Early development of P. umbilicalis from neutral spores is mostly linear. Blades bearing neutral spores were frozen (-20 ºC) for 4 weeks to determine if freezing influences early development. Freezing had minor effects on early development compared to the rate and pattern of germination from NS in untreated controls. Attempted genetic transformation of P. umbilicalis using biolistics was successful, but whether it was a stable transformation is unknown; hygromycin B was demonstrated to be an effective antibiotic to use in selection of transformants. Use of scanning electron microscopy (SEM) revealed that microbial communities on the surface of P. umbilicalis are often diverse, complex, and vary between different groups of specimens. Together, these studies support continuing development of P. umbilicalis as a model organism and a valuable aquaculture crop.
Royer, Charlotte, "Advancing Development of Porphyra umbilicalis as a Red Algal Model System and Aquaculture Crop" (2017). Electronic Theses and Dissertations. 2683.