Additional Participants

Graduate Student

Sophia Tang
Guixiong Zhong
Anusha Ramanajam
Robert Baron
Michael Steeves

Undergraduate

Peter Buchanan

Technician, Programmer

Andrew Wallace
Mike Call

Undergraduate

Sarah Langeley

Organizational Partners

Sandia Laboratories-Livermore

Project Period

September 1, 2003-August 31, 2005

Level of Access

Open-Access Report

Grant Number

0352401

Submission Date

8-8-2006

Abstract

This project investigates a new technique for in situ quantification of Polymerase Chain Reaction (PCR) amplification products using electrochemiluminescence (ECL). The technique implements the highly sensitive, ECL detection and quantification of tris(2,2'bipyridyl) ruthenium (II) labeled DNA. This method is expected to yield significant improvement in speed, cost and performance over existing quantitative PCR methods, by reducing the number and quantity of reagents, reducing the number of sample preparation steps, increasing sensitivity, and shortening analysis time.

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