Author

Pei-Yu Chen

Date of Award

5-2009

Level of Access Assigned by Author

Campus-Only Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Biochemistry and Molecular Biology

Advisor

Robert Friesel

Second Committee Member

Lucy Liaw

Third Committee Member

Douglas Spicer

Abstract

Vascular smooth muscle cells (VSMCs) play a critical role in the physiological maintenance of the cardiovascular system. In contrast to skeletal and cardiac muscle cells, VSMCs have the capacity to modulate their phenotype from contractile to synthetic and proliferative states. This phenotypic switch plays an important role in pathological processes, including hypertension, atherosclerosis, and restenosis after angioplasty. Understanding the molecular mechanism of how VSMCs modulate their phenotype is an important step in the understanding of cardiovascular disease. My studies have focused on examining the mechanism of VSMC phenotypic modulation by fibroblast growth factor receptor-1 (FGFRl). I have developed a set of FGFRl pathway-deficient mutants to examine the signaling pathway(s) mediated by FGFRl that control VSMC phenotype. My results showed that the FGFRl-mediated FRS2 pathway, but not the Crk or PLCγ pathways, is required for regulating VSMC phenotype. This is the first reported evidence that FRS2 participates in VSMC phenotypic modulation. In addition to FGF, platelet-derived growth factor (PDGF) is a well documented growth factor that regulates switching of VSMC from a contractile phenotype to proliferative and synthetic phenotype. I found that FGF2 and PDGF-BB modulate VSMC phenotype including increase cell proliferation and decrease smooth muscle marker gene expression. I also found that FGFRl co-immunoprecipitated with PDGFRβ. This complex formation does not require receptor activation and is mediated through both the extracellular and intracellular domains. Moreover, FRS2 knockdown in VSMC reversed FGF2 and PDGF-BB mediated downregulation of smooth muscle marker gene expression; while leaving PDGF-BB-induced ERK activation and cell proliferation intact. FRS2 is an adaptor protein linking FGFR to downstream MAP kinase and Akt/mTOR signaling. My biochemical studies showed that mTOR is a downstream target of FGFR1/FRS2. FGFRl activation phosphorylated mTOR at Ser2448 site and mTOR inhibitor rapamycin partially reversed FGFRl-mediated VSMC phenotypic switch by increasing SM a-actin protein synthesis. In addition, mTOR was co-immunoprecipitated with activated FGFR1/FRS2. In summary, the results of this study will provide information on FGFRl signaling in VSMC phenotypic switch and also set the stage for future design of new drugs that may selectively block FGFRl signaling for pharmacological interventions.

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